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Updated: May 15, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
Published on: February 8, 2014
Noise and signal scaling factors in digital holography in weak illumination: relationship with shot noise
M Lesaffre1, N Verrier, M Gross
1Institut Langevin: Unité Mixte de Recherche (UMR) 7587 Centre National de la Recherche Scientifique (CNRS), Ecole Supérieure de Physique et de Chimie Industrielle de Paris (ESPCI), ParisTech, 1 rue Jussieu, Paris 75005, France.
Abstract:
We have performed off-axis heterodyne holography with very weak illumination by recording holograms of the object with and without object illumination in the same acquisition run. We have experimentally studied how the reconstructed image signal (with illumination) and noise background (without) scale with the holographic acquisition and reconstruction parameters that are the number of frames and the number of pixels of the reconstruction spatial filter. The first parameter is related to the frequency bandwidth of detection in time, the second one to the bandwidth in space. The signal to background ratio varies roughly like the inverse of the bandwidth in time and space. We have also compared the noise background with the theoretical shot-noise background calculated by Monte Carlo simulation. The experimental and Monte Carlo noise background agree very well with each other.
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