Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

<i>HK2</i> knockdown is associated with context-dependent inflammatory and angiogenesis-related transcriptional programmes in glioblastoma cells.

Frontiers in immunology·2026

Same author

Comprehensive reconstruction of the musculoskeletal anatomy in the shoulder using a hybrid 3D ultrasound mosaicking workflow: A pilot study.

PloS one·2026

Same author

Istaroxime's Effect on Hemodynamics and Arrhythmias in Cardiogenic Shock: A Pooled Analysis of SEISMiC Trials.

JACC. Heart failure·2026

Same author

Value frameworks for digital health technologies: a comparative analysis.

Journal of medical economics·2026

Same author

From ChatGPT to UroGPT: A guideline-trained artificial intelligence model for male infertility.

Current urology·2026

Same author

Lipid droplets in neurodegenerative diseases: pathological drivers and therapeutic vulnerabilities.

Cell death discovery·2026

Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026

Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026

Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026

Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026

Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026

Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 15, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Fast phase reconstruction in white light diffraction phase microscopy.

Hoa V Pham¹, Christopher Edwards, Lynford L Goddard

¹Quantitative Light Imaging Laboratory, Department of Electrical and Computer Engineering, Beckman Institute for Advanced Science & Technology, University of Illinois at Urbana–Champaign, Urbana, Illinois 61801, USA.

|January 8, 2013

Summary

This summary is machine-generated.

This study introduces a new technique for white light diffraction phase microscopy that eliminates computationally intensive phase unwrapping. This method significantly speeds up quantitative phase imaging, enabling real-time applications like blood testing.

More Related Videos

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Related Experiment Videos

Last Updated: May 15, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Area of Science:

Optical Physics
Biomedical Imaging
Microscopy Techniques

Background:

Off-axis interferometry often requires computationally intensive phase unwrapping.
Phase wrapping errors occur in thick samples and due to non-zero beam interference angles.
Real-time phase reconstruction is hindered by the unwrapping process.

Purpose of the Study:

To propose a technique that bypasses the phase unwrapping step in quantitative phase imaging.
To enable faster phase reconstruction in white light diffraction phase microscopy.
To facilitate high-impact applications, including real-time blood testing.

Main Methods:

Developed a simplified technique for quantitative phase reconstruction.
Applied the method to white light diffraction phase microscopy of thin samples.
Eliminated the need for traditional phase unwrapping algorithms.

Main Results:

Achieved significant improvement in phase reconstruction speed.
Demonstrated the avoidance of the phase unwrapping step.
Enabled real-time phase imaging capabilities.

Conclusions:

The proposed technique offers a faster alternative for quantitative phase reconstruction.
This method removes a major bottleneck in interferometric imaging.
Opens possibilities for real-time biomedical diagnostics and analysis.