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Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

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Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes
08:34

Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes

Published on: September 27, 2018

Mycobacterium abscessus multispacer sequence typing.

Mohamed Sassi1, Imen Ben Kahla, Michel Drancourt

  • 1Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, Faculté de médecine, 27, Boulevard Jean Moulin-Cedex 5, Marseille, France.

BMC Microbiology
|January 9, 2013
PubMed
Summary
This summary is machine-generated.

Multispacer sequence typing (MST) accurately identifies and differentiates Mycobacterium abscessus subspecies. This new method aids in distinguishing M. abscessus strains, crucial for understanding and treating infections caused by these antibiotic-resistant bacteria.

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Area of Science:

  • Microbiology
  • Genetics
  • Infectious Diseases

Background:

  • The Mycobacterium abscessus group comprises antibiotic-resistant, opportunistic bacteria causing various infections.
  • Accurate strain discrimination within this group is challenging due to close genetic relationships.

Purpose of the Study:

  • To develop and evaluate a multispacer sequence typing (MST) analysis for simultaneous identification and genotyping of M. abscessus.
  • To compare the performance of MST with the established multilocus sequence analysis (MLSA) typing method.

Main Methods:

  • Selection and sequencing of eight intergenic spacers based on the M. abscessus CIP104536T genome.
  • Application of MST to 21 M. abscessus isolates and 48 available M. abscessus genomes.
  • Comparison of MST results with MLSA and rpoB gene sequencing.

Main Results:

  • MST and MLSA successfully grouped M. abscessus organisms into distinct types, with MST yielding 12 types and MLSA nine.
  • The discriminatory power, measured by the Hunter-Gaston index, was high for both methods (MST: 0.912, MLSA: 0.903).
  • MST spacer n°2 sequencing alone proved sufficient for accurate sub-species level identification.

Conclusions:

  • Multispacer sequence typing (MST) is an effective sequencing-based approach for identifying and genotyping M. abscessus.
  • MST clearly differentiates M. massiliense from other M. abscessus subsp. bolletii organisms.
  • This method offers a valuable tool for the precise characterization of M. abscessus strains.