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Related Experiment Videos

Prostaglandin-E2 released by pre-implantation human conceptuses.

P V Holmes1, A Sjögren, L Hamberger

  • 1Department of Obstetrics and Gynecology, University of Göteborg, Sweden.

Journal of Reproductive Immunology
|March 1, 1990
PubMed
Summary

Human conceptuses produce prostaglandin E2 (PGE2), an immune suppressor, throughout early development. Late blastocysts produced the most PGE2, especially in media with bovine serum albumin.

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Area of Science:

  • Reproductive biology
  • Immunology
  • Prostaglandin research

Background:

  • Prostaglandin E2 (PGE2) is a key regulator of immune cell function.
  • Understanding PGE2 production during human pre-implantation development is crucial for reproductive success.
  • Cumulus cells, surrounding the oocyte, may influence conceptus development.

Purpose of the Study:

  • To investigate prostaglandin E2 (PGE2) production by human pre-implantation conceptuses at various developmental stages.
  • To determine the source of PGE2 production, distinguishing between conceptus and cumulus cells.
  • To assess the impact of culture media composition on PGE2 synthesis.

Main Methods:

  • Human conceptuses (4-cell to blastocyst stages) and cumulus oophorus cells were obtained from IVF/ET programs.

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  • Cells and conceptuses were cultured for 48 hours.
  • PGE2 levels in culture media were quantified.
  • Indomethacin was used to confirm synthesis by conceptuses.
  • PGF2a production was also assessed.

    • Main Results:

    • Cumulus cell complexes produced significant amounts of PGE2.
    • All stages of human conceptuses produced PGE2, with highest levels from late blastocysts.
    • PGE2 production was greater in media containing bovine serum albumin compared to human donor serum.
    • Indomethacin treatment confirmed PGE2 synthesis by conceptuses.
    • No significant PGF2a production was detected from human blastocysts.

    Conclusions:

    • Human pre-implantation conceptuses synthesize and release PGE2.
    • PGE2 production increases with conceptus developmental stage, peaking at the blastocyst stage.
    • Culture conditions, including serum type and the presence of cumulus cells, influence PGE2 production.