Related Experiment Video
Updated: May 15, 2026

Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
Published on: December 26, 2011
Characterizing the glycosylation state of therapeutic recombinant glycoproteins
Nicole Samuels1, David Kates, Jun Liu
1Analytical Development and Support, Global Biological Development, Bayer Healthcare LLC, Berkeley, CA, USA. nicole.samuels@bayer.com
Abstract:
As an increasing number of recombinant therapeutic glycoproteins are manufactured and investigated, the importance of their attached glycans is becoming more widely reported and understood. Regulatory agencies expect detailed "extended characterization" of the glycoprotein as well as routine, well-controlled "release assays" with specifications to be employed for quality control of each manufactured lot. In this chapter we will briefly discuss relevant glycan issues in the area of therapeutic recombinant glycoprotein manufacture and describe in detail two assays that are employed in the development of, for example, recombinant Factor VIII for the treatment of hemophilia.
Related Concept Videos
Protein Glycosylation
Glycosylation occurs in...
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Proteoglycans
Protein Folding Quality Check in the RER
