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Updated: May 15, 2026
Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry
Published on: September 13, 2022
Chemiluminescent detection of senescence-associated β galactosidase
Vinicius Bassaneze1, Ayumi Aurea Miyakawa, José Eduardo Krieger
1Laboratory of Genetics and Molecular Cardiology/LIM 13, Heart Institute (InCor), University of Sao Paulo Medical School, Sao Paulo, Brazil.
Abstract:
Identifying molecules that serve as markers for cell aging is a goal that has been pursued by several groups. Senescence-associated β galactosidase (SA-βgal) staining is broadly used and very easily detected. β-gal is a lysosomal enzyme strongly correlated to the progression of cell senescence. Here, we describe a simple, fast, and quantitative protocol to quantify SA-βgal activity in cell lysate extracts by a chemiluminescent method using galacton as substrate.
