Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
Loss of Carboxy Group as CO2: Decarboxylation of β-Ketoacids01:02

Loss of Carboxy Group as CO2: Decarboxylation of β-Ketoacids

Carboxylic acids, upon heating, undergo a decarboxylation reaction by releasing carbon dioxide gas. Monocarboxylic acids do not undergo decarboxylation easily. However, a silver salt of carboxylic acid reacts with bromine or iodine under high temperature to release carbon dioxide gas and forms halide with one less carbon. This reaction is called the Hunsdiecker reaction.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mutation of a single cysteine in CaMKIIδ protects the heart from ischemia-reperfusion Injury.

bioRxiv : the preprint server for biology·2026
Same author

Double alkylation with maleimide-PEG-biotin: An enrichment method for cysteine redox states.

Analytical biochemistry·2025
Same author

Metabolic dependency mapping identifies Peroxiredoxin 1 as a driver of resistance to ATM inhibition.

Redox biology·2025
Same author

Oxidation of CaMKIIα cysteines inhibits autonomous activation induced by phosphorylation.

Archives of biochemistry and biophysics·2024
Same author

Corrigendum to "Loss of methionine sulfoxide reductases increases resistance to oxidative stress" [Free Radic. Biol. Med. 145 (2019) 374-384].

Free radical biology & medicine·2024
Same author

Lactate oxidation in Paracoccus denitrificans.

Archives of biochemistry and biophysics·2024

Related Experiment Video

Updated: May 15, 2026

Synthesizing Amino Acids Modified with Reactive Carbonyls in Silico to Assess Structural Effects Using Molecular Dynamics Simulations
05:57

Synthesizing Amino Acids Modified with Reactive Carbonyls in Silico to Assess Structural Effects Using Molecular Dynamics Simulations

Published on: April 26, 2024

Quantification of protein carbonylation.

Nancy B Wehr1, Rodney L Levine

  • 1Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 9, 2013
PubMed
Summary

This study details methods for measuring protein carbonylation, a key indicator of oxidative stress. Protocols for spectrophotometric and immunochemical assays using 2,4-dinitrophenylhydrazine (DNPH) are provided, including a sensitive dot blot technique.

More Related Videos

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
12:49

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry

Published on: April 4, 2018

Quantification of Coenzyme A in Cells and Tissues
08:51

Quantification of Coenzyme A in Cells and Tissues

Published on: September 27, 2019

Related Experiment Videos

Last Updated: May 15, 2026

Synthesizing Amino Acids Modified with Reactive Carbonyls in Silico to Assess Structural Effects Using Molecular Dynamics Simulations
05:57

Synthesizing Amino Acids Modified with Reactive Carbonyls in Silico to Assess Structural Effects Using Molecular Dynamics Simulations

Published on: April 26, 2024

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
12:49

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry

Published on: April 4, 2018

Quantification of Coenzyme A in Cells and Tissues
08:51

Quantification of Coenzyme A in Cells and Tissues

Published on: September 27, 2019

Area of Science:

  • Biochemistry
  • Oxidative Stress Research
  • Proteomics

Background:

  • Protein carbonylation is a significant marker of oxidative protein damage.
  • Quantification of protein carbonylation is crucial for understanding cellular responses to oxidative stress.

Purpose of the Study:

  • To present optimized protocols for measuring protein carbonylation.
  • To detail derivatization and quantification methods using 2,4-dinitrophenylhydrazine (DNPH).
  • To introduce a highly sensitive dot blot assay for protein carbonylation.

Main Methods:

  • Spectrophotometric assay for protein carbonylation.
  • Immunochemical assay for protein carbonylation.
  • Dot blot assay utilizing 2,4-dinitrophenylhydrazine (DNPH) derivatization.

Main Results:

  • Established protocols for spectrophotometric and immunochemical quantification of protein carbonylation.
  • Demonstrated a novel dot blot method with enhanced sensitivity for detecting protein carbonylation.

Conclusions:

  • The presented protocols provide reliable methods for assessing protein carbonylation.
  • The enhanced dot blot assay offers a sensitive tool for oxidative stress research.