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Updated: May 15, 2026

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
Published on: May 26, 2013
Complete genome sequence analysis of an American isolate of Grapevine virus E
Olufemi J Alabi1, Sudarsana Poojari, Kara Sarver
1Department of Plant Pathology, Irrigated Agriculture Research and Extension Center, Washington State University, Prosser, WA 99350, USA.
Abstract:
The complete genome sequence of Grapevine virus E (GVE) collected from a red-berried wine grape cultivar (Cabernet Sauvignon) in Washington State was determined. The 7,568 nucleotide long genome of GVE is more similar in sequence identity with a GVE isolate from a wine grape cv. Shiraz from South Africa when compared with an isolate from "Aki Queen" grape from Japan. Like GVE isolates from South Africa and Japan, the Washington isolate encodes five open reading frames (ORFs) and the overall genome organization is identical among these isolates. In addition to AlkB domain, a DExD domain, belonging to the DEAD-like helicases superfamily, was present upstream of the helicase domain in the replicase ORF of the virus.
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