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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: May 15, 2026

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

Studying microRNAs in lymphoma.

Joost Kluiver¹, Izabella Slezak-Prochazka, Anke van den Berg

¹Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands. j.l.kluiver@umcg.nl

Methods in Molecular Biology (Clifton, N.J.)

|January 9, 2013

Summary

This study details methods for investigating microRNAs (miRNAs) in lymphoma. Researchers can now assess miRNA expression, overexpress miRNAs, and determine their oncogenic or tumor-suppressive roles in lymphomagenesis.

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Area of Science:

Molecular Biology
Oncology
Genetics

Background:

MicroRNAs (miRNAs) are crucial regulators of cellular processes, including development and differentiation.
Dysregulation of miRNAs is implicated in various diseases, notably lymphoma.
Understanding miRNA functions is vital for advancing lymphoma research and treatment.

Purpose of the Study:

To describe robust methodologies for studying microRNA involvement in lymphoma.
To provide protocols for quantitative assessment and functional analysis of miRNAs in lymphomagenesis.
To facilitate the investigation of specific miRNAs as potential therapeutic targets or biomarkers in lymphoma.

Main Methods:

Multiplex reverse transcription (RT) followed by quantitative polymerase chain reaction (qPCR) for differential miRNA expression analysis.
Lentiviral-based ectopic expression systems for stable miRNA overexpression in lymphoma cells.
A functional assay to ascertain whether a candidate miRNA acts as an oncogene or a tumor suppressor in lymphomagenesis.

Main Results:

Established protocols enable precise quantification of candidate miRNA expression levels.
Successfully demonstrated stable overexpression of miRNAs using lentiviral vectors.
Developed a method to functionally characterize miRNAs as oncogenes or tumor suppressors in the context of lymphoma.

Conclusions:

The described methods provide a comprehensive toolkit for exploring the role of miRNAs in lymphoma.
These techniques will aid in identifying novel miRNA biomarkers and therapeutic strategies for lymphoma.
This work empowers researchers to elucidate the specific functions of miRNAs in lymphomagenesis.