Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Growth of Cartilage and Bone Tissue01:27

Growth of Cartilage and Bone Tissue

Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measurement of chondrocyte chemotaxis using a Boyden chamber: a model of receptor-mediated cell migration combined with cell sedimentation.

Mathematical medicine and biology : a journal of the IMA·2012
Same author

Analysis of collagen and glucose modulated cell growth within tissue engineered scaffolds.

Annals of biomedical engineering·2010
Same author

Hybrid cellular automaton modeling of nutrient modulated cell growth in tissue engineering constructs.

Journal of theoretical biology·2009
Same author

The effect of cell sedimentation on measuring chondrocyte population migration using a Boyden chamber.

Journal of theoretical biology·2009
Same author

A compact computational model for cell construct development in perfusion culture.

Biotechnology and bioengineering·2007
Same author

Enhancement of cell growth in tissue-engineering constructs under direct perfusion: Modeling and simulation.

Biotechnology and bioengineering·2007

Related Experiment Video

Updated: May 15, 2026

Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering
08:04

Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering

Published on: April 25, 2013

Computational modeling of nutrient utilization in engineered cartilage.

Tze-Hung Lin1, Han-Yun Jhang, Feng-Cheng Chu

  • 1Dept. of Mechanical Engineering, Army Academy ROC, Jhongli, 32092, Taiwan, ROC.

Biotechnology Progress
|January 9, 2013
PubMed
Summary

A new mathematical model explains how oxygen levels can rise in chondrocyte cultures. High glucose increases acidity, slowing metabolism and oxygen use, leading to oxygen accumulation in tissue engineering scaffolds.

More Related Videos

Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants
08:42

Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants

Published on: January 7, 2019

An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants
07:10

An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants

Published on: November 3, 2019

Related Experiment Videos

Last Updated: May 15, 2026

Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering
08:04

Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering

Published on: April 25, 2013

Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants
08:42

Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants

Published on: January 7, 2019

An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants
07:10

An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants

Published on: November 3, 2019

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Tissue Engineering

Background:

  • Chondrocytes are crucial for cartilage maintenance and repair.
  • In vitro chondrocyte culture is vital for tissue engineering.
  • Understanding nutrient dynamics in cell culture is essential for optimizing growth.

Purpose of the Study:

  • To develop a mathematical model for simulating chondrocyte culture in scaffolds.
  • To investigate the impact of glucose, oxygen, and pH on chondrocyte metabolic rates.
  • To explain previously observed increases in oxygen concentration during static chondrocyte culture.

Main Methods:

  • Development of a mathematical model for chondrocyte-seeded scaffolds.
  • Simulation of cell metabolism, nutrient consumption, and waste production.
  • Analysis of the effects of varying glucose and oxygen concentrations and pH levels.

Main Results:

  • The model explains how increased acidity from high glucose metabolism can lead to rising oxygen levels in scaffolds.
  • High glucose conditions result in increased lactate production, higher acidity, and reduced cell metabolic rates.
  • Low glucose conditions lead to a continuous decrease in oxygen concentration due to slower glycolysis.
  • The study reveals complex correlations between nutrient uptake, lactate secretion, and pH changes.

Conclusions:

  • The mathematical model provides insights into the dynamic changes of oxygen and pH in chondrocyte cultures.
  • The findings offer a reference for optimizing in vitro cell culture conditions in tissue engineering.
  • Understanding these metabolic interactions is key to advancing cartilage tissue regeneration strategies.