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Updated: May 15, 2026

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays
Published on: March 14, 2011
Short communication: nuclear JAK3 and its involvement in CD4 activation in HIV-infected patients
Ivan Landires1, Virginia Núñez-Samudio, Jacques Thèze
1Unité d'Immunogénétique Cellulaire, Département Infection et Epidémiologie et Département d'Immunologie, Institut Pasteur, Paris, France. ivanlandires@yahoo.es
Abstract:
The subcellular localization of JAK3 was examined by quantitative image analysis. For the first time, JAK3 was found to be located in the nuclei of primary CD4 lymphocytes. A comparable quantity of JAK3 was recovered in CD4 lymphocytes from healthy donors and HIV-infected patients. By contrast, far more phosphorylated JAK3 (pJAK3) was found in the nuclei of CD4 lymphocytes from HIV-infected patients than from healthy donors. The correlation detected between the quantity of pJAK3 in the nuclei of CD4 lymphocytes and the increase in HLA-DR at their surface suggests that pJAK3 may play a role in the deleterious immune activation characterizing HIV-infected patients.
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