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10:42
Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
Supragingival plaque microbial analysis in reflection to caries experience.
Alaa Mannaa1, Anette Carlén, Guglielmo Campus
1Department of Cariology, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, Box 450, Gothenburg, SE-405 30, Sweden. alaa.mannaa@odontologi.gu.se
BMC Oral Health
|January 10, 2013
Summary
Pooled plaque samples analyzed with checkerboard DNA-DNA hybridization did not correlate with caries experience in children and mothers. This method, unlike previous saliva analysis, failed to establish a significant relationship between bacterial counts and tooth decay levels.
Area of Science:
- Oral microbiology
- Dental diagnostics
Background:
- Dental caries is caused by cariogenic bacteria metabolizing carbohydrates in biofilms.
- Previous studies suggested saliva analysis could reflect caries experience.
Purpose of the Study:
- To determine if pooled supragingival plaque bacteria quantified by checkerboard DNA-DNA hybridization correlate with caries experience.
- To compare this method with previous saliva-based chair-side analyses.
Main Methods:
- 86 mothers and their children (4-6 and 12-16 years) participated.
- Caries experience (DMFT/dmft) was assessed clinically and radiographically (D3 level).
- Pooled supragingival plaque samples were analyzed for 15 bacterial species using checkerboard DNA-DNA hybridization.
Main Results:
- No significant relationships were found between bacterial scores and DMFT/dmft.
- No significant associations were observed between bacterial counts and caries severity groups (D/d).
Conclusions:
- Checkerboard DNA-DNA hybridization of pooled plaque samples did not correlate with caries experience in this cohort.
- This contrasts with previous findings using saliva samples and chair-side methods.
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