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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

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Related Experiment Video

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Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
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Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

Dip-and-read method for label-free renewable sensing enhanced using complex DNA structures.

Min Zhang, Xiao-Qin Jiang, Huynh-Nhu Le

    ACS Applied Materials & Interfaces
    |January 10, 2013
    PubMed
    Summary

    This study introduces a novel label-free assay for sensitive DNA detection using DNA tetrahedrons and biolayer interferometry, improving detection limits 100-fold. The method is adaptable for detecting ATP, offering new possibilities for biosensing applications.

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    Area of Science:

    • Biotechnology
    • Nanotechnology
    • Biosensing

    Background:

    • Label-free detection methods are crucial for sensitive biomolecule analysis.
    • Biolayer interferometry (BLI) offers label-free detection but can be limited by signal amplification.
    • Complex DNA nanostructures can be engineered to enhance optical signals.

    Purpose of the Study:

    • To develop a highly sensitive label-free assay for DNA detection.
    • To leverage DNA tetrahedrons for signal amplification in biolayer interferometry.
    • To explore the adaptability of this assay for detecting other biomolecules, such as ATP.

    Main Methods:

    • Utilized complex DNA structures (DNA tetrahedrons) to amplify optical signals.
    • Employed biolayer interferometry (BLI) for label-free detection.
    • Demonstrated target-dependent adaptability using aptamers for ATP detection.

    Main Results:

    • Achieved a 100-fold improvement in the detection limit for DNA.
    • Successfully adapted the assay for adenosine triphosphate (ATP) detection.
    • Established a robust and sensitive label-free detection platform.

    Conclusions:

    • The developed DNA tetrahedron-enhanced BLI assay significantly improves DNA detection sensitivity.
    • This label-free biosensing approach is versatile and can be extended to detect other targets like ATP.
    • The findings present new opportunities for developing advanced biolayer interferometry-based assays.