The formation of occlusion-derived virus is affected by the expression level of ODV-E25

Odv-e25 is a core gene of baculoviruses and encodes a 25.5 kDa protein located on both budded virus (BV) and occlusion-derived virus (ODV). Our previous study demonstrated that ODV-E25 was required for the formation of intranuclear microvesicles and ODV, and an odv-e25 deletion mutant could be rescued by re-expression of odv-e25 under its native promoter. To investigate the functions of ODV-E25 expression level on ODV formation, the promoter of ie-1 (pIE1), the odv-e25 native promoter, and the polyhedrin promoter (pPH) were used to direct odv-e25 expression. Our results showed that the production of ODV-E25 under its native promoter was higher than that under pIE1 but lower than that under pPH. Viral DNA replication and budded viruses (BVs) production showed that expression of odv-e25 under pIE1 and pPH could not completely repair the defects caused by the deletion of ODV-E25, while expression under its native promoter did. Electron microscopy showed that intranuclear microvesicles were found in all the constructs transfected cells except the odv-e25-null virus. However, mature ODVs only were detected in cells transfected with virus in which odv-e25 was expressed under its native or polyhedrin promoter. These results indicated that the formation occlusion-derived virus was affected by the expression level of ODV-E25.