Microscopic analysis of ovule development in Arabidopsis thaliana

Ovules are the major female reproductive organs in higher plants. In addition, ovules of Arabidopsis thaliana are successfully used as model system to study plant organogenesis. Here we describe two microscopic techniques to analyze Arabidopsis ovule development from the organ to the cellular level in a rapid and reproducible fashion. Both methods are of great value when comparing the morphology of wild-type and mutant ovule development.