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Updated: May 15, 2026

Hairy Root Transformation and Regeneration in Arabidopsis thaliana and Brassica napus
Published on: December 22, 2023
Regeneration in Arabidopsis tissue culture
Kaoru Sugimoto1, Elliot M Meyerowitz
1Division of Biology 156-29, California Institute of Technology, Pasadena, CA, USA.
Abstract:
An entire Arabidopsis plant can be regenerated from a small piece of tissue by two sequential hormonal treatments in tissue culture. Currently this in vitro regeneration system is a good system to study the mechanism by which plants show regenerative plasticity. Also, it is useful to test the hormone sensitivity of plants and to propagate sterile lines in Arabidopsis. Here we describe a standard protocol for regenerating Arabidopsis plants in tissue culture, and for preparing and observing samples using confocal microscopy to study cells during regeneration.
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