Related Experiment Video
Updated: May 15, 2026
Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
Published on: October 2, 2017
Direct determination of polymyxin B sulfate using resonance Rayleigh scattering and resonance non-linear scattering
Ling Kong1, Zhongfang Liu, Shaopu Liu
1School of Chemistry, Chemical Engineering, Key Laboratory of Luminescence, Real-time Analysis of the Ministry of Education, Southwest University, Chongqing, China.
Abstract:
At pH 1.3-1.6, tungstate WO4(2-) , can be converted to hexatungstate W6 O19(2-) , which can react with positively charged polymyxin B sulfate (PMB) to result in enhancement of resonance Rayleigh scattering (RRS) and resonance non-linear scattering, including second order scattering and frequency doubling scattering. Linear relationships can be established between enhanced scattering intensity and PMB concentration. The detection limits (3σ) were 5.5 ng/mL (RRS), 10.1 ng/mL (second order scattering) and 34.6 ng/mL (frequency doubling scattering). The optimum reaction conditions, influencing factors and related analytical properties were tested. The interaction mechanism was investigated via absorption spectrum, circular dichroism spectra and atomic force microscopy imaging. The basis of scattering enhancement is discussed. PMB in eardrops, human serum and urine, were quantified satisfactorily by RRS.
