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Updated: May 15, 2026

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
09:24

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Published on: September 13, 2018

Single cell network profiling assay in bladder cancer.

Todd M Covey1, Manish A Vira, Matt Westfall

  • 1Research, Nodality, Inc., South San Francisco, CA 94080, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|January 10, 2013
PubMed
Summary
This summary is machine-generated.

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This study shows single cell network profiling (SCNP) can analyze rare bladder cancer cells from washings. The assay successfully identified signaling in cancer cells, demonstrating its potential for disease characterization and drug development.

Area of Science:

  • Oncology
  • Cell Biology
  • Biotechnology

Background:

  • Bladder cancer (BC) diagnosis and treatment monitoring often require invasive procedures.
  • Analyzing rare epithelial cancer cells in patient samples presents significant technical challenges.
  • Understanding signaling pathways in cancer cells is crucial for targeted therapy development.

Purpose of the Study:

  • To evaluate the feasibility of the single cell network profiling (SCNP) assay for examining signaling networks in rare epithelial cancer cells.
  • To develop and refine methods for detecting, distinguishing, and quantifying signaling in cancer cells from bladder washings.
  • To assess the potential of SCNP as a tool for bladder cancer characterization and drug development.

Main Methods:

  • Utilized multiparametric flow cytometry with antibodies (CD45, cytokeratin, EpCAM, cPARP) to identify and isolate epithelial cells.

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Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
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Last Updated: May 15, 2026

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
09:24

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Published on: September 13, 2018

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
09:45

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

  • Employed DAPI staining to measure DNA content and determine aneuploidy for distinguishing tumor from normal cells.
  • Assessed PI3K and MAPK pathway activity by measuring p-AKT and p-ERK levels, including responses to pathway modulators and epidermal growth factor.

    • Main Results:

    • The SCNP assay was successfully applied to bladder washings from 29 BC and 15 non-BC subjects.
    • 66% of BC samples met evaluability criteria, allowing for robust signaling analysis.
    • SCNP identified quantifiable increases in p-AKT and p-ERK in cancer cells from four BC samples, with pathway-specific responses to inhibitors.

    Conclusions:

    • The SCNP assay is a feasible method for the functional characterization of rare bladder cancer cells.
    • This technique enables reproducible quantification of signaling pathways in low-frequency cancer cells.
    • Standardized SCNP could advance bladder cancer disease characterization and support drug development efforts.