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Both Myosin-10 isoforms are required for radial neuronal migration in the developing cerebral cortex
Xing-Da Ju1, Ye Guo, Nan-Nan Wang
1Key Laboratory of Molecular Epigenetics, Ministry of Education, Institute of Genetics and Cytology, Northeast Normal University, Changchun 130021, China.
Cerebral Cortex (New York, N.Y. : 1991)
|January 10, 2013
Summary
Myosin-10 (Myo10) isoforms guide developing cortical neurons. Full-length Myo10 orients migration, while headless Myo10 aids shape changes, ensuring proper brain development.
Area of Science:
- Neuroscience
- Developmental Biology
- Cell Biology
Background:
- During mammalian cerebral cortex development, postmitotic neurons migrate radially from the ventricular zone to the cortical plate.
- This radial migration is crucial for forming functional neural circuits and requires precise control over neuronal orientation and morphology.
- Myosin motors are essential for various cellular processes, including cell migration and morphogenesis.
Purpose of the Study:
- To investigate the distinct roles of the two Myosin-10 (Myo10) isoforms in regulating radial neuronal migration in the developing mouse cerebral cortex.
- To elucidate the molecular mechanisms underlying the functions of each Myo10 isoform in neuronal migration and morphogenesis.
Main Methods:
- Utilized mouse models to study Myosin-10 isoform expression and localization during cortical development.
- Employed techniques to assess neuronal migration, orientation, and morphology.
- Investigated the involvement of the netrin-1 receptor deleted in colorectal cancer (DCC) in Myo10-mediated migration.
Main Results:
- The full-length Myo10 (fMyo10) isoform is localized in deeper cortical layers and is essential for establishing correct radial migration orientation.
- fMyo10-dependent orientation is partly mediated by the netrin-1 receptor DCC.
- The headless Myo10 (hMyo10) isoform is required in the intermediate zone for the transition from multipolar to bipolar neuronal morphology.
Conclusions:
- The study reveals divergent and essential functions for the two Myo10 isoforms in mammalian cortical development.
- fMyo10 regulates the directionality of radial neuronal migration, while hMyo10 controls neuronal shape transition.
- These findings highlight the complex regulation of neuronal migration and morphogenesis by Myo10 isoforms.
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