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Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
Published on: February 14, 2022
Rapid clinical bacteriology and its future impact.
Alex van Belkum1, Géraldine Durand, Michel Peyret
1BioMérieux SA, Unit Microbiology, R&D Microbiology, La Balme Les Grottes, France.
Annals of Laboratory Medicine
|January 10, 2013
Summary
Clinical microbiology is slowly advancing, with culture-based methods being replaced by nucleic acid and mass spectrometry technologies for bacterial identification. However, antibiotic susceptibility testing requires further innovation.
Area of Science:
- Clinical microbiology
- Bacteriology
- Diagnostic technologies
Background:
- Clinical bacteriology has historically relied on culture-based methods for over a century.
- Integration of serological and molecular techniques occurred gradually.
- Nucleic acid amplification technologies and mass spectrometry have recently revolutionized microbial identification.
Purpose of the Study:
- To review recent advancements in laboratory-based clinical bacteriology.
- To provide an overview of emerging novel diagnostic approaches.
- To highlight areas needing improvement, such as antibiotic susceptibility testing.
Main Methods:
- Review of literature on clinical bacteriology advancements.
- Analysis of the impact of new diagnostic technologies.
- Discussion of emerging diagnostic strategies.
Main Results:
- Culture-based methods are being supplanted by nucleic acid detection and mass spectrometry for microbial identification.
- These technological shifts represent a paradigm shift in diagnostic microbiology.
- Significant progress has been made in microbial identification, but antibiotic susceptibility testing lags.
Conclusions:
- Clinical microbiology is undergoing modernization driven by new technologies and emerging pathogens.
- Mass spectrometry is becoming a new gold standard for microbial identification.
- Further development is crucial for improving microbial antibiotic susceptibility testing.
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