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Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry
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Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry

Published on: January 12, 2018

Red blood cell deformability is very slightly decreased in erythropoietin deficient mice

Aurélien Pichon1, Yann Lamarre2, Nicolas Voituron1

  • 1Université Paris 13, Sorbonne Paris Cité, Laboratoire «Réponses cellulaires et fonctionnelles à l'hypoxie» EA2363, Bobigny, France.

Clinical Hemorheology and Microcirculation
|January 11, 2013
PubMed

The present study compared the hemorheological properties between Epo-TAgh mice (a model of erythropoietin deficient mice) and wild-type (WT) control mice. Blood viscosity was determined at several shear rates using a cone-plate viscometer at native and adjusted hematocrit (i.e. 40%). Red blood cell (RBC) deformability was measured by ecktacytometry at several shear stresses and RBC aggregation properties by backscattered technique at adjusted hematocrit (i.e. 40%). Epo-TAgh mice had severe anemia (very low hematocrit), decreased blood viscosity at native hematocrit and slightly reduced RBC deformability at high shear stresses in comparison with WT mice. Blood viscosity at adjusted hematocrit (i.e. 40%) was not different between WT and Epo-TAgh mice. RBC aggregation did not differ between the two mice models. These findings suggest a role of erythropoietin in the regulation of RBC deformability.

Keywords:
Erythropoietinanemiahemorheology

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