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Updated: May 15, 2026

Collection and Extraction of Saliva DNA for Next Generation Sequencing
Published on: August 27, 2014
Small RNA transcriptomes of two types of exosomes in human whole saliva determined by next generation sequencing
Yuko Ogawa1, Yoshitaka Taketomi, Makoto Murakami
1Faculty of Pharmaceutical Sciences, Teikyo Heisei University, Ichihara, Chiba 290–0193, Japan.
Abstract:
Small non-coding RNAs, such as microRNAs (miRNAs), are involved in diverse processes, including organ development and tissue differentiation. Exosomes are small membrane vesicles (30-100 nm in diameter) produced by numerous cells. Recently, exosomes have been shown to contain miRNAs. However, the small RNAs contained in exosomes are not fully characterized. In a previous study, we found at least two types of salivary exosome that are different in size and have different proteomes. Studies of salivary exosomal small RNAs are limited to miRNAs. In this study, we examined small RNA transcriptomes using next generation sequencing technology to elucidate a full transcriptome set of small RNAs expressed in the two types of salivary exosomes and in whole saliva (WS). Many types of small RNA, such as miRNA, piwi-interacting RNA (piRNA), small nucleolar RNA (snoRNA) and other small RNAs are contained in salivary exosomes and WS. Among these small RNAs we identified novel miRNA candidates.
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