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Updated: May 15, 2026
A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Published on: March 9, 2017
Chemiluminescence detection of glucose using Ce(IV) oxidation in a batch system
Megumi Nagai1, Wakayo Miyahara, Honami Sagawa
1Separation Science Division, Faculty of Science, Okayama University of Science, Okayama, Japan.
Abstract:
A chemiluminescence method for the determination of glucose (GC) using a batch system made in our laboratory is proposed. The method is based on measures of the chemiluminesence intensity from a batch of sample GC solutions following the injection of an (NH(4))(2)[Ce(NO(3))(6)]-[Ru(bpy)(3)]Cl(2) solution. The effect of the Ru complex concentration on chemiluminescence was studied. The detection limit was 0.5 × 10(-8) M, and the linear calibration curve reached 10(-7) M. The Ru complex was selected on the basis of its desirable performance compared to those of other photosensitizers. However, flow-injection analysis (FIA) using the Ce-Ru complex reagent was not applicable for the detection of GC.
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