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Sebastian Fabritz1, Sebastian Hörner, Olga Avrutina

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Cube-octameric silsesquioxanes (COSS) are versatile scaffolds for creating bioconjugates. Their low toxicity and aqueous solubility make them promising for drug delivery and diagnostics.

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Bioconjugation Chemistry

Background:

  • Cube-octameric silsesquioxanes (COSS) are increasingly recognized for their potential as nanoparticle scaffolds.
  • Their compact, symmetric structure offers a unique platform for molecular design.

Purpose of the Study:

  • To explore the utility of COSS as scaffolds for tailor-made bioconjugates.
  • To highlight the diverse applications enabled by functionalized COSS nanoparticles.

Main Methods:

  • Utilizing conjugation chemistries such as copper-catalyzed azide-alkyne cycloaddition (CuAAC), thiol-ene-click (TEC), and oxime ligation.
  • Decorating COSS nanoparticles with up to eight addressable organic substituents.

Main Results:

  • Successful conjugation of various biorelevant ligands, including carbohydrates, peptides, miniproteins, reporter molecules, and rare-earth chelates.
  • Demonstration of COSS-based molecules' low toxicity and solubility in aqueous systems.
  • Confirmation of sufficient half-life for in vivo studies.

Conclusions:

  • COSS nanoparticles serve as effective scaffolds for creating sophisticated bioconjugates.
  • The versatility in ligand attachment and favorable physicochemical properties position COSS for significant applications in drug delivery, tumor diagnostics, and therapy.