Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

PGI2 and PGD2 effects on cyclic AMP and human T-cell mitogenesis.

S W Burchiel

    Prostaglandins and Medicine
    |November 1, 1979
    PubMed
    Summary

    Prostaglandins PGD2 and PGE2 suppress T cell proliferation by increasing cyclic AMP in human peripheral blood lymphocytes (HPBL). PGI2 is less effective due to instability, impacting cyclic AMP levels and mitogenesis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Complete Genome Sequence of a Novel WU Polyomavirus Isolate from Arkansas, USA, Associated with Acute Respiratory Infection.

    Genome announcements·2017
    Same author

    Complete Genome Sequences of Two Novel Isolates of Human Parainfluenza Virus 1 Associated with Acute Respiratory Infection.

    Genome announcements·2016
    Same author

    Mechanisms for how inhaled multiwalled carbon nanotubes suppress systemic immune function in mice.

    Nature nanotechnology·2009
    Same author

    Prevention of apoptosis by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the MCF-10A cell line: correlation with increased transforming growth factor alpha production.

    Cancer research·2001
    Same author

    A bioactive metabolite of benzo[a]pyrene, benzo[a]pyrene-7,8-dione, selectively alters microsomal Ca2+ transport and ryanodine receptor function.

    Molecular pharmacology·2001
    Same author

    Analysis of genetic and epigenetic mechanisms of toxicity: potential roles of toxicogenomics and proteomics in toxicology.

    Toxicological sciences : an official journal of the Society of Toxicology·2001

    Area of Science:

    • Immunology
    • Biochemistry
    • Cell Biology

    Background:

    • Prostaglandins (PGs) are lipid compounds with diverse physiological roles.
    • Cyclic AMP (cAMP) is a crucial second messenger in cellular signaling.
    • Human peripheral blood lymphocytes (HPBL) play a key role in immune responses.

    Purpose of the Study:

    • To investigate the effects of PGI2, PGD2, and PGF2 alpha on HPBL cyclic AMP levels.
    • To determine the impact of these prostaglandins on T cell mitogen response (PHA).
    • To elucidate the relationship between cAMP modulation and lymphocyte proliferation suppression.

    Main Methods:

    • Measurement of intracellular cyclic AMP levels in HPBL.
    • Assessment of HPBL proliferation in response to phytohemagglutinin (PHA).
    • Comparative analysis of prostaglandin effects on cAMP and proliferation.

    Main Results:

    • PGI2, PGD2, and PGE2 all increased intracellular cAMP levels in HPBL.
    • Only PGD2 and PGE2 significantly suppressed PHA-induced lymphocyte proliferation.
    • PGI2 showed reduced efficacy in maintaining elevated cAMP and suppressing mitogenesis, potentially due to instability.

    Conclusions:

    • PGD2 and PGE2 are potent suppressors of T cell mitogenesis, mediated by elevated cAMP.
    • PGI2's instability limits its ability to suppress lymphocyte proliferation.
    • PGD2 mimics PGE2's effects on both cAMP levels and T cell mitogenesis suppression.

    Related Experiment Videos