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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
Published on: July 12, 2013
Multiplex mass spectrometry imaging for latent fingerprints.
Gargey B Yagnik1, Andrew R Korte, Young Jin Lee
1USDOE-Ames Laboratory, Ames, IA 50011, USA.
Journal of Mass Spectrometry : JMS
|January 11, 2013
Summary
Multiplex mass spectrometry imaging (MSI) enables rapid chemical analysis of latent fingerprints. This technique provides rich chemical data from limited samples, identifying both endogenous and exogenous compounds.
Area of Science:
- Analytical Chemistry
- Forensic Science
- Mass Spectrometry Imaging
Background:
- Matrix-assisted laser desorption/ionization MS imaging (MSI) offers detailed chemical information.
- Previous work developed in-parallel data acquisition combining Orbitrap and ion trap mass spectrometry (MS) for faster MSI.
- Limited sample amounts often pose challenges in chemical analysis.
Purpose of the Study:
- To demonstrate a novel application of multiplex MSI for latent fingerprint analysis.
- To showcase the ability to acquire rich chemical information from fingerprints in a single experiment.
- To validate the utility of multiplex MSI for analyzing trace chemical evidence.
Main Methods:
- Utilized in-parallel data acquisition combining Orbitrap MS and ion trap MS/MS scans.
- Applied multiplex MSI methodology to latent fingerprint samples.
- Acquired simultaneous chemical images and tandem MS/MS (MS/MS) images in one experiment.
Main Results:
- Successfully obtained chemical images of diverse endogenous and exogenous compounds from latent fingerprints.
- Generated simultaneous MS/MS images for selected compounds within the same imaging run.
- Demonstrated the efficiency of multiplex MSI in capturing comprehensive chemical profiles.
Conclusions:
- Multiplex MSI is a powerful tool for comprehensive chemical analysis of latent fingerprints.
- The methodology significantly reduces data acquisition time while enhancing chemical information richness.
- This approach is particularly valuable when dealing with limited biological or forensic specimens.
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