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Updated: May 15, 2026

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Published on: August 5, 2016

Pathogenic brucellae replicate in human trophoblasts.

Suzana Pinto Salcedo¹, Nicolas Chevrier, Thais Lourdes Santos Lacerda

¹Aix-Marseille Université UM 2, Centre d'Immunologie de Marseille-Luminy.

The Journal of Infectious Diseases

|January 11, 2013

Summary

Brucella bacteria infect human trophoblasts, replicating within these cells and potentially breaching the fetal-maternal barrier. Different Brucella species utilize distinct cellular compartments, impacting early pregnancy outcomes.

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In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

Published on: March 5, 2019

Area of Science:

Microbiology
Cell Biology
Reproductive Biology

Background:

Brucellae are intracellular pathogens known to replicate within host cells, often utilizing endoplasmic reticulum (ER)-derived vacuoles.
Trophoblasts are crucial for pregnancy, forming the fetal-maternal barrier, and are known targets for Brucella in animals.

Purpose of the Study:

To investigate the susceptibility and replication dynamics of Brucella species within human trophoblasts.
To determine the specific intracellular replication niches utilized by different Brucella species in trophoblasts.
To assess the impact of Brucella infection on the invasive capacity of extravillous trophoblasts (EVTs).

Main Methods:

Infection of immortalized and primary human trophoblasts with Brucella species.
Microscopic analysis to identify intracellular replication compartments (ER-derived vs. lysosomal).
In vitro assays to evaluate the effect of Brucella infection on EVT invasiveness.

Main Results:

Brucella species extensively proliferated within various human trophoblast subpopulations.
B. abortus and B. suis replicated in lysosomal membrane-associated protein 1 (LAMP-1)-positive inclusions.
B. melitensis replicated in an ER-derived compartment and impaired EVT invasive capacity.

Conclusions:

Human trophoblasts serve as a significant replication niche for Brucella, especially when the fetal-maternal barrier is compromised.
The distinct replication strategies of Brucella species in trophoblasts, particularly B. melitensis's effect on EVT invasion, may play a critical role in Brucella-associated pregnancy loss.