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The Blood-brain Barrier00:49

The Blood-brain Barrier

Overview

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Related Experiment Video

Updated: May 15, 2026

Reconstruction of the Blood-Brain Barrier In Vitro to Model and Therapeutically Target Neurological Disease
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Reconstruction of the Blood-Brain Barrier In Vitro to Model and Therapeutically Target Neurological Disease

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Modeling the blood-brain barrier using stem cell sources.

Ethan S Lippmann1, Abraham Al-Ahmad, Sean P Palecek

  • 1Dept, of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Dr,, Madison, WI, 53706, USA. shusta@engr.wisc.edu.

Fluids and Barriers of the CNS
|January 12, 2013
PubMed
Summary
This summary is machine-generated.

Stem cells offer new ways to model the blood-brain barrier (BBB) in vitro, aiding research into its development, function, and neurological diseases. This approach overcomes limitations of traditional models for drug screening.

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Improved Method for the Preparation of a Human Cell-based, Contact Model of the Blood-Brain Barrier

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A Triple Culture Cell System Modeling the Human Blood-Brain Barrier
09:21

A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

Published on: November 30, 2021

Area of Science:

  • Neuroscience
  • Cell Biology
  • Stem Cell Research

Background:

  • The blood-brain barrier (BBB) is crucial for brain homeostasis, regulating substance transport.
  • BBB development involves complex cellular interactions, making in vivo studies challenging.
  • Existing in vitro BBB models face limitations in cell availability and fidelity, especially from human sources.

Purpose of the Study:

  • To review the application of stem cells in modeling blood-brain barrier (BBB) development and function.
  • To explore the use of neural progenitor cells (NPCs) for studying BBB induction and adult BBB co-culture models.
  • To present the differentiation of human pluripotent stem cells (hPSCs) into BBB-characteristic endothelial cells for disease modeling and drug screening.

Main Methods:

  • Utilizing neural progenitor cells (NPCs) to investigate BBB induction.
  • Employing differentiated NPCs in co-culture systems to model the adult BBB.
  • Differentiating human pluripotent stem cells (hPSCs) into endothelial cells exhibiting BBB properties.

Main Results:

  • NPCs can be used to study early BBB induction processes.
  • Co-cultures with differentiated NPCs effectively model aspects of the adult BBB.
  • hPSC-derived endothelial cells demonstrate robust BBB characteristics, suitable for advanced modeling.

Conclusions:

  • Stem cell-derived models provide powerful tools to overcome limitations in studying the BBB.
  • These advanced in vitro models facilitate research on BBB development, neurological diseases, and neurotherapeutics.
  • hPSC-based BBB models hold significant promise for drug discovery and personalized medicine.