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Related Concept Videos

RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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Dual core processing: MRB1 is an emerging kinetoplast RNA editing complex.

Hassan Hashimi1, Sara L Zimmer, Michelle L Ammerman

  • 1Institute of Parasitology, Biology Center, Czech Academy of Sciences, Faculty of Science, University of South Bohemia, České Budějovice (Budweis) 370 05, Czech Republic.

Trends in Parasitology
|January 12, 2013
PubMed
Summary

The mitochondrial RNA-binding complex 1 (MRB1) is vital for kinetoplastid RNA (kRNA) editing. Our model shows MRB1 coordinates guide RNA exchange for multi-round editing and links it to other RNA processing events.

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Kinetoplastid RNA editing relies on guide RNAs (gRNAs) and the RNA editing core complex (RECC) for uridine insertion/deletion in mitochondrial mRNAs.
  • The mitochondrial RNA-binding complex 1 (MRB1) has been identified as crucial for this editing process, but its precise role remains unclear.

Purpose of the Study:

  • To elucidate the function of the mitochondrial RNA-binding complex 1 (MRB1) in kinetoplastid RNA editing.
  • To integrate functional data of MRB1 subunits within the context of its overall architecture.
  • To propose a model for MRB1's involvement in coordinating RNA editing and processing.

Main Methods:

  • Characterization of constituent subunits of the mitochondrial RNA-binding complex 1 (MRB1).
  • Elucidation of the MRB1 complex architecture.
  • Development of a functional model for MRB1 based on structural and biochemical data.

Main Results:

  • The study provides a structural understanding of the MRB1 complex.
  • A model is proposed where MRB1 mediates multi-round kinetoplastid RNA editing by managing guide RNA (gRNA) exchange for the RNA editing core complex (RECC).
  • MRB1 is implicated in linking kinetoplastid RNA editing with downstream RNA processing events.

Conclusions:

  • The MRB1 complex plays a critical dual role in kinetoplastid RNA metabolism.
  • MRB1 facilitates efficient editing of long mRNA regions by coordinating multiple gRNA-RECC interactions.
  • MRB1 integrates RNA editing with other essential RNA processing steps, ensuring proper gene expression in kinetoplastids.