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Particle Agglutination Method for Poliovirus Identification
07:06

Particle Agglutination Method for Poliovirus Identification

Published on: April 20, 2011

Quantitative one-step RT-PCR assay for rapid and sensitive identification and titration of polioviruses in clinical

Majid Laassri1, Anthony Dipiazza, Bella Bidzhieva

  • 1Center for Biologics Evaluation and Research, US Food and Drug Administration, 1401 Rockville Pike, HFM 470, Rockville, MD 20852-1448, USA. majid.laassri@fda.hhs.gov

Journal of Virological Methods
|January 12, 2013
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...