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Updated: May 15, 2026

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
Published on: September 7, 2022
Lymphocytes and B-cell abnormalities in patients with common variable immunodeficiency (CVID)
L Berrón-Ruiz1, G López-Herrera2, A Vargas-Hernández3
1Department of Molecular Biomedicine, Center for Research and Advanced Studies, IPN, Avenida Instituto Politécnico Nacional # 2508, Colonia Zacatenco, 07360 Mexico DF, Mexico; Immunodeficiency Research Unit, National Institute of Pediatrics SSA, Avenida Insurgentes Sur # 3700-C, Colonia Insurgentes Cuicuilco, Mexico City, Mexico; Laboratory of Immunochemistry I, National School of Biological Sciences, IPN, Carpio y Plan de Ayala s/n, Colonia Santo Tomas, 11340 Mexico DF, Mexico.
Background And Aims:
Common variable immunodeficiency (CVID) is a primary antibody deficiency characterised by decreased antibody production and low or normal B-cell numbers. To elucidate the clinical and immunological heterogeneity of CVID, we studied 16 patients diagnosed with CVID.
Methods:
We analysed B, T and NK cell populations. We also assessed CD27 expression to define B-cell subsets and examined the expression of molecules important in B-cell proliferation and differentiation, such as the transmembrane activator and CALM interactor (TACI), inducible costimulator (ICOS), CD154 and CD40.
Results:
We observed reduced B and T-cell numbers in CVID patients; this reduction was more pronounced in adults. While one group of patients (group I) showed a significant reduction in CD27+ memory B-cells, another group (group II) of patients exhibited numbers of CD27+ memory B-cells similar to the healthy donor. The frequency of B-cells and T-cells expressing CD40 and ICOS, respectively, was significantly lower in all CVID patients compared with healthy donors. Finally, a correlation between the frequency of CD27+ memory B-cells and clinical features was observed in CVID patients.
Conclusion:
These results suggest that in some patients, the combined defects in both T and B-cells may account for CVID. Additionally, patients in group I exhibited an increased frequency of pneumonia and chronic diarrhoea.
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