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Related Experiment Video

Updated: May 15, 2026

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

Simple automated liquid chromatographic system for splitless nano column gradient separations.

Jozef Sesták¹, Filip Duša, Dana Moravcová

¹Faculty of chemistry, Brno University of Technology, Purkyňova 464/118, 612 00 Brno, Czech Republic.

Journal of Chromatography. A

|January 12, 2013

Summary

This summary is machine-generated.

A new liquid chromatography system for micro/nano separations creates reproducible gradients using a syringe pump. This system offers high repeatability for retention times and peak areas in complex sample analyses.

Related Experiment Videos

Last Updated: May 15, 2026

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

Area of Science:

Analytical Chemistry
Chromatography

Background:

Micro and nano column chromatography require precise mobile phase gradients.
Existing systems can be complex and expensive.

Purpose of the Study:

To develop and test a simple, cost-effective gradient liquid chromatography system for micro/nano separations.
To evaluate the system's ability to generate reproducible gradients and injections.

Main Methods:

Assembly of a system using a programmable syringe pump and a ten-port valve for gradient formation via turbulent mixing.
Verification of gradient profiles using uracil as a marker with varying syringe volumes (50-250 μl).
Separation of alkylphenones and tryptic digests on a capillary monolithic column.

Main Results:

The system successfully generated uniform water-acetonitrile gradients with up to 80% linearity.
High repeatability of retention times (RSD < 0.3%) was achieved.
Excellent repeatability of peak areas (RSD < 5%) was demonstrated for small injection volumes (0.1-3 μl) using on-column preconcentration.

Conclusions:

The developed splitless gradient liquid chromatography system is suitable for micro and nano column applications.
The system provides high precision and reproducibility, making it a valuable tool for sensitive analyses.