Related Experiment Video
Updated: May 15, 2026

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
Published on: October 26, 2016
Methoxyethylammonium monosubstituted β-cyclodextrin as the chiral selector for enantioseparation in capillary
Shuye Wang1, Yun Dai, Jianhua Wu
1Key Laboratory of Soft Chemistry and Functional Materials, Ministry of Education, Nanjing University of Science and Technology, Nanjing 210094, China.
Abstract:
Methoxyethylamine monosubstituted β-cyclodextrin, mono-6(A)-(2-methoxyethyl-1-ammonium)-6(A)-β-cyclodextrin chloride (MEtAMCD), is synthesized and analytically characterized. Bearing a methoxy group in cyclodextrin rim, MEtAMCD exhibits outstanding enantioselectivities toward ampholytic and acidic racemates in capillary electrophoresis. Driven by inclusion complexation, electrostatic interactions and/or hydrogen bonding, the enantioseparation of MEtAMCD is found to be strongly dependent on various separation parameters including buffer pH, cyclodextrin concentration, applied voltage, separation temperature and organic solvent additives. MEtAMCD demonstrates as a versatile cationic chiral selector for the studied 26 acidic and ampholytic enantiomers.
More Related Videos
14:53Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
Published on: January 16, 2017
08:56Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
Published on: November 22, 2024
Related Concept Videos
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Ion-Exchange Chromatography
Electrophoresis: Overview
There...
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
Capillary Electrophoresis: Instrumentation
Prochirality