Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

Maintenance of the ES Cell State

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

Source And Potency Of Stem Cells

Source And Potency Of Stem Cells

Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...

Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

ZNF512B safeguards genome integrity at regulatory regions to repress the SASP and inflammation.

Cell stem cell·2026

Same author

Mesenchymal drift: A convergent framework for the hallmarks of aging.

Cell·2026

Same author

Vitamin C inhibits ACSL4 to alleviate ferro-aging in primates.

Cell metabolism·2026

Same author

Reprogramming the GRHL2-CDK19 axis by gene therapy alleviates prostate aging.

Nature aging·2025

Same author

Vitamin C conveys geroprotection on primate ovaries.

Cell stem cell·2025

Same author

Prevalent mesenchymal drift in aging and disease is reversed by partial reprogramming.

Cell·2025

Search research articles

Related Experiment Video

Updated: May 15, 2026

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency

Published on: June 10, 2018

Characterization of pluripotent stem cells.

Mercè Martí¹, Lola Mulero, Cristina Pardo

¹Center of Regenerative Medicine in Barcelona, Barcelona, Spain.

Nature Protocols

|January 12, 2013

Summary

This summary is machine-generated.

Standardized immunodetection protocols quickly characterize pluripotent stem cells for reliable cell line registration. These methods ensure consistent quality and comparability across diverse stem cell sources and species.

More Related Videos

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

Related Experiment Videos

Last Updated: May 15, 2026

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency

Published on: June 10, 2018

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

Area of Science:

* Stem Cell Biology
* Molecular Biology
* Immunology

Background:

* Characterization of pluripotent stem cells is essential for stem cell line registration and objective comparison of generated lines.
* Establishing specific, fast, and reliable protocols is crucial for detecting pluripotency hallmarks.
* Existing methods may lack standardization, leading to variations in results.

Purpose of the Study:

* To describe standardized immunodetection protocols for characterizing mouse and human pluripotent stem cell lines.
* To provide reliable and fast methods for detecting key markers of pluripotency and differentiation.
* To ensure consistent and comparable results across different experimental conditions.

Main Methods:

* Immunocytochemistry for detecting pluripotency markers (2 days).
* Immunocytochemistry for identifying three germ layers in embryoid bodies (3 days).
* Immunohistochemistry for detecting differentiation markers in teratomas (4 days).

Main Results:

* Developed standardized protocols minimizing variations due to cell source, species, or sample quantity.
* Achieved fast and high-quality results for stem cell characterization.
* Enabled reliable assessment of pluripotency and differentiation potential.

Conclusions:

* Standardized immunodetection protocols are vital for accurate and efficient characterization of pluripotent stem cells.
* These protocols facilitate objective comparison and registration of stem cell lines.
* The described methods offer a reliable framework for stem cell research and application.