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Discrimination of isobaric Leu/Ile residues by MALDI in-source decay mass spectrometry
Daiki Asakawa1, Nicolas Smargiasso, Edwin De Pauw
1Chemistry Department and GIGA-R, Mass Spectrometry Laboratory, University of Liege, Liege, Belgium. dasakawa@yokohama-cu.ac.jp
Abstract:
MALDI in-source decay (ISD) has been used for top-down sequencing of proteins. The use of the matrix 1,5-diaminonapthalene (1,5-DAN) gave abundant w ions, which are formed from the unimolecular dissociation of z• radical fragments via α cleavage reaction and thus help identify which of the isobaric amino acids, Leu or Ile, is present. The high abundance of w ions in MALDI-ISD with 1,5-DAN results from the low collision rate in the MALDI plume. MALDI-ISD with 1,5-DAN appears to be an useful method for the top-down sequencing of proteins, including discrimination of Leu and Ile near the C-terminal end.
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