Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microtubules in Signaling01:22

Microtubules in Signaling

The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hyperosmolality activates polycystin-2 and TRPM4 in renal primary cilium.

Pflugers Archiv : European journal of physiology·2024
Same author

Regenerative Calcium Currents in Renal Primary Cilia.

Frontiers in physiology·2022
Same author

Inward Ca<sup>2+</sup> current through the polycystin-2-dependent channels of renal primary cilia.

American journal of physiology. Renal physiology·2021
Same author

The TRPP2-dependent channel of renal primary cilia also requires TRPM3.

PloS one·2019
Same author

Primary cilia regulate the osmotic stress response of renal epithelial cells through TRPM3.

American journal of physiology. Renal physiology·2017
Same author

The native TRPP2-dependent channel of murine renal primary cilia.

American journal of physiology. Renal physiology·2016

Related Experiment Video

Updated: May 15, 2026

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
07:53

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Published on: January 16, 2018

A method for measuring electrical signals in a primary cilium.

Nancy K Kleene1, Steven J Kleene

  • 1Department of Cancer and Cell Biology, University of Cincinnati, PO Box 670521, Cincinnati, OH 45267-0521, USA.

Cilia
|January 12, 2013
PubMed
Summary

Researchers developed a new method for electrical recordings from primary cilia, enabling study of their function in sensory transduction and disease. This technique allows direct measurement of ion channel activity within cilia.

More Related Videos

Efficient and Cost Effective Electroporation Method to Study Primary Cilium-Dependent Signaling Pathways in the Granule Cell Precursor
04:06

Efficient and Cost Effective Electroporation Method to Study Primary Cilium-Dependent Signaling Pathways in the Granule Cell Precursor

Published on: November 30, 2021

Volumetric Imaging and Analysis of Primary Cilia in Musculoskeletal Tissue using the ARL13B-CENTRIN-2 Mouse Model
09:53

Volumetric Imaging and Analysis of Primary Cilia in Musculoskeletal Tissue using the ARL13B-CENTRIN-2 Mouse Model

Published on: March 28, 2025

Related Experiment Videos

Last Updated: May 15, 2026

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
07:53

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Published on: January 16, 2018

Efficient and Cost Effective Electroporation Method to Study Primary Cilium-Dependent Signaling Pathways in the Granule Cell Precursor
04:06

Efficient and Cost Effective Electroporation Method to Study Primary Cilium-Dependent Signaling Pathways in the Granule Cell Precursor

Published on: November 30, 2021

Volumetric Imaging and Analysis of Primary Cilia in Musculoskeletal Tissue using the ARL13B-CENTRIN-2 Mouse Model
09:53

Volumetric Imaging and Analysis of Primary Cilia in Musculoskeletal Tissue using the ARL13B-CENTRIN-2 Mouse Model

Published on: March 28, 2025

Area of Science:

  • Cell Biology
  • Biophysics
  • Neuroscience

Background:

  • Most cells have a primary cilium, crucial for sensory stimuli transduction.
  • Ciliary defects are linked to various diseases.
  • Understanding ciliary electrical properties is vital but challenging due to their small size.

Purpose of the Study:

  • To develop a novel method for sensitive and repeatable electrical recordings from primary cilia.
  • To investigate the electrical properties and ion channel activity within primary cilia.

Main Methods:

  • Cells were cultured on spherical beads for easy manipulation.
  • Primary cilia were isolated and pulled into a recording microelectrode using suction.
  • Excised cilia allowed for controlled solution bathing and electrical recording.

Main Results:

  • Successful high-resistance seals were achieved in 47% of attempts.
  • Single channel currents were recorded from intact and excised cilia.
  • Ionic currents were modulated by cytoplasmic Ca(2+) and transmembrane voltage.

Conclusions:

  • Ciliary recording provides a direct method to study transduction channels.
  • This technique facilitates understanding the effects of second messengers and voltage on ciliary function.