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Related Experiment Video

Updated: May 15, 2026

Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice
08:01

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Published on: May 16, 2021

AMPKα1: a glucose sensor that controls CD8 T-cell memory.

Julia Rolf1, Marouan Zarrouk, David K Finlay

  • 1Division of Cell Signalling and Immunology, University of Dundee, Dundee, Scotland, UK.

European Journal of Immunology
|January 12, 2013
PubMed
Summary
This summary is machine-generated.

Adenosine monophosphate-activated protein kinase (AMPK)α1 monitors T cell energy stress. AMPKα1 is crucial for CD8 T cell survival and the generation of robust CD8 T cell memory responses.

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06:07

Measuring Mitochondrial Function of Naïve and Effector CD8 T Cells

Published on: March 28, 2025

Area of Science:

  • Immunology
  • Cellular Metabolism
  • Molecular Biology

Background:

  • T cells, crucial for adaptive immunity, undergo metabolic shifts during immune responses.
  • Adenosine monophosphate-activated protein kinase (AMPK) is a key regulator of cellular energy homeostasis.
  • AMPK activation in T cells is linked to antigen receptor signaling and metabolic stress.

Purpose of the Study:

  • To investigate the role of AMPK in the metabolic transition of effector CD8 T cells to memory T cells.
  • To determine if AMPK regulates CD8 T cell survival and memory formation during the contraction phase of the immune response.

Main Methods:

  • Analysis of AMPKα1 activation in CD8 cytotoxic T lymphocytes (CTLs) under glucose deprivation.
  • Assessment of mammalian target of rapamycin complex 1 (mTORC1) activity in response to glucose stress.
  • Evaluation of CTL survival and memory CD8 T-cell generation in AMPKα1(null) mice during Listeria monocytogenes infection.

Main Results:

  • AMPKα1 rapidly activates in CD8 CTLs experiencing glucose deprivation-induced metabolic stress.
  • AMPKα1 restrains mTORC1 activity under conditions of glucose stress.
  • AMPKα1 is essential for in vivo survival of CTLs after immune stimulation withdrawal and for generating memory CD8 T cell responses.

Conclusions:

  • AMPKα1 acts as an energy stress sensor in CD8 T cells.
  • AMPKα1 plays a critical role in regulating CD8 T cell survival and the establishment of CD8 T cell memory.