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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
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Aeromonas aquariorum clinical isolates: antimicrobial profiles, plasmids and genetic determinants

Suat-Moi Puah1, S D Puthucheary, Fong-Yin Liew

  • 1Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

International Journal of Antimicrobial Agents
|January 15, 2013
PubMed

Insights

This study examined Aeromonas aquariorum clinical isolates, finding high resistance to amoxicillin/clavulanic acid and amoxicillin. The bacteria carried plasmids and specific antibiotic resistance genes (ARGs), contributing to understanding their resistance mechanisms.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Aeromonas aquariorum is an emerging pathogen.
  • Understanding antimicrobial resistance (AMR) in clinical isolates is crucial for effective treatment.
  • Plasmids and antibiotic resistance genes (ARGs) play a significant role in AMR.

Purpose of the Study:

  • To investigate antimicrobial resistance patterns in clinical Aeromonas aquariorum isolates.
  • To identify the presence of plasmids and associated antibiotic resistance genes (ARGs).

Main Methods:

  • Antibiotic susceptibility testing using the disc diffusion method.
  • Plasmid detection via gel electrophoresis.
  • Identification of ARGs using monoplex PCR.

Main Results:

  • High resistance rates observed for amoxicillin/clavulanic acid (98%) and amoxicillin (91%).
  • Lower resistance to gentamicin (13%), trimethoprim/sulfamethoxazole (11%), and kanamycin (6%).
  • No resistance detected for ciprofloxacin or amikacin.
  • All isolates harbored plasmids (2-10 kb) and carried bla(TEM), bla(MOX), bla(PSE), sul1, and sul2 genes.

Conclusions:

  • Clinical Aeromonas aquariorum isolates exhibit significant multidrug resistance.
  • The presence of specific β-lactam and sulfonamide resistance genes on plasmids contributes to the observed resistance phenotype.
  • This research enhances understanding of the genetic basis for AMR in Aeromonas aquariorum.

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