Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A new approach to immunoFET operation.

R B Schasfoort1, R P Kooyman, P Bergveld

  • 1Department of Applied Physics, University of Twente, Enschede, The Netherlands.

Biosensors & Bioelectronics
|January 1, 1990
PubMed
Summary

A novel method uses ion diffusion in a membrane to detect immunological reactions via ISFET measurements. This technique directly quantifies protein charge density and isoelectric points, simplifying biomolecule analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Outcome of the geriatric head and neck cancer patient].

HNO·2026
Same author

Exoscopic-assisted head and neck surgery: a comparative evaluation optimizing ergonomics and workflow.

Journal of robotic surgery·2025
Same author

[Typical emergencies in otorhinolaryngology-a monocentric analysis of the seasonal course].

HNO·2022
Same author

[Oncologic follow-up in head and neck cancer patients].

HNO·2022
Same author

Performance of microvascular anastomosis with a new robotic visualization system: proof of concept.

Journal of robotic surgery·2021
Same author

[Robotics in otorhinolaryngology, head and neck surgery].

HNO·2021

Area of Science:

  • Biosensors
  • Analytical Chemistry
  • Immunochemistry

Background:

  • Detecting immunological reactions often requires complex labeling and washing steps.
  • Understanding macromolecular charge properties is crucial for biosensor development.
  • Existing methods may not directly measure charge density or isoelectric points of adsorbed proteins.

Purpose of the Study:

  • To present a new method for detecting immunological reactions in a membrane using an ISFET.
  • To enable direct measurement of immobile charge density and isoelectric points of proteins adsorbed in a membrane.
  • To correlate protein concentration in solution with membrane concentration.

Main Methods:

  • Utilizing an Ion-Selective Field-Effect Transistor (ISFET) to measure transient membrane potentials.

Related Experiment Videos

  • Inducing transient ion diffusion through a membrane-protein layer by altering electrolyte concentration.
  • Analyzing the relationship between diffusion rate, immobile charge density, and antibody-antigen complex formation.

    • Main Results:

    • A transient membrane potential is generated and measured by the ISFET, correlating with protein charge density.
    • Isoelectric points of embedded proteins are determined by identifying the zero potential response.
    • The method successfully studied membrane adsorption of lysozyme and human serum albumin (HSA), including HSA-anti-HSA immune reactions.
    • An empirical equation describes the influence of protein parameters on transient amplitude, and Langmuir behavior correlates solution and membrane protein concentrations.

    Conclusions:

    • This novel ISFET-based method allows for direct, label-free detection of immunological reactions.
    • It provides unique capabilities for measuring charge densities and isoelectric points of adsorbed amphoteric macromolecules.
    • The simplified procedure offers direct insights into specific charge properties of studied macromolecules.