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Updated: May 15, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Next-generation sequencing technologies and their impact on microbial genomics.

Brian M Forde¹, Paul W O'Toole

¹Department of Microbiology, University College Cork, Cork, Ireland. pwotoole@ucc.ie.

Briefings in Functional Genomics

|January 15, 2013

Summary

Next-generation sequencing offers a cost-effective, high-throughput method for microbial genomics. These advanced technologies enable comprehensive analysis of microbial genomes, surpassing previous capabilities.

Keywords:

NGS RNA-seq genome sequencing metagenomics prokaryotes resequencing

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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

Area of Science:

Genomics
Microbiology
Bioinformatics

Background:

Traditional capillary sequencing methods are being replaced by advanced next-generation sequencing (NGS) technologies.
NGS provides a low-cost, high-throughput alternative, revolutionizing genomic research.
The scope of NGS has expanded beyond initial applications, offering diverse utility-based analyses.

Purpose of the Study:

To discuss the current applications of next-generation sequencing methods in microbial genome research.
To explore the impact and contributions of NGS to the field of microbial genomics.
To provide an outlook on the imminent commercialization of third-generation sequencing technologies.

Main Methods:

Review of current next-generation sequencing technologies.
Analysis of utility-based applications in microbial genome research.
Discussion of the impact of NGS on microbial genomics.

Main Results:

NGS enables more comprehensive analysis of microbial genome structure and content.
These technologies have significantly advanced the field of microbial genomics.
The transition to third-generation sequencing is expected to further enhance capabilities.

Conclusions:

Next-generation sequencing has profoundly impacted microbial genome research.
The versatility of NGS allows for unprecedented depth in genomic analysis.
Future sequencing technologies promise even greater advancements in understanding microbial life.