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Related Experiment Videos

Deuterium relaxation and internal motion in solid Li-DNA.

R Brandes1, R R Vold, D R Kearns

  • 1Department of Chemistry, University of California-San Diego, La Jolla 92093.

Biochemistry
|February 20, 1990
PubMed
Summary

Internal motion in DNA was studied using spectral densities. Results suggest uniaxial librational motion of the C8-D bond, ruling out torsional motion as the primary mechanism at lower hydration levels.

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Area of Science:

  • Molecular Biophysics
  • Solid-State Nuclear Magnetic Resonance (NMR)
  • Biopolymers

Background:

  • Understanding internal molecular motion is crucial for polynucleotide function.
  • Deuterated DNA samples provide specific sites for NMR relaxation studies.
  • Hydration significantly influences DNA dynamics.

Purpose of the Study:

  • To investigate the nature of internal motion in oriented, hydrated DNA.
  • To measure spectral densities of motion J1(omega 0) and J2(2 omega 0).
  • To determine the dominant relaxation mechanisms and motional models.

Main Methods:

  • Solid-state deuterium NMR spectroscopy on oriented, partially hydrated calf thymus Li-DNA.
  • Deuteration at the guanine and adenine 8-positions.

Related Experiment Videos

  • Measurement of spectral densities J1 and J2 at varying hydration levels.

    • Main Results:

    • Spectral densities J1 and J2 increase with hydration.
    • J1 is 2-5 times larger than J2, with frequency dependencies of omega-1 and omega-3/2.
    • High J1/J2 ratios indicate uniaxial librational motion of the C8-D bond (correlation times 0.2-3.0 µs) below 10 H2O/nucleotide.

    Conclusions:

    • In-plane torsional motion is not the dominant relaxation mechanism at low hydration.
    • A decrease in the J1/J2 ratio at high hydration suggests increasing torsional motion contributions.
    • The C8-D bond exhibits approximately uniaxial librational motion under specific hydration conditions.