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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
Stabilization, purification and crystallization of catalytic subunit of cAMP-dependent protein kinase from bovine
1Department of Biochemistry, Asahikawa Medical College, Japan.
Biochimica Et Biophysica Acta
|April 19, 1990
Abstract:
The catalytic subunit of cAMP-dependent protein kinase purified from bovine heart was significantly stabilized by non-ionic detergents. Freezing and thawing did not cause a significant decrease in the enzyme activity in the presence of 0.1% Tween 80. A very convenient method for purification and crystallization of the catalytic subunit was also described.
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