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The expression of moesin in astrocytoma: correlation with pathologic grade and poor clinical outcome
Ming Wu1, Ding-Yang Liu, Xian-Rui Yuan
1Department of Neurosurgery, Xiangya Hospital, Central South University, The Institute of Skull Base Surgery and Neurooncology at Hunan, 87 Xiangya Road, Changsha 410008, Hunan, China.
Abstract:
Moesin, a member of the ERM family, acts as a linker between the actin cytoskeleton and the plasma membrane and plays a key role in the control of cell morphology, motility, adhesion and other processes of tumourigenesis. The expression pattern and clinical significance of moesin in astrocytoma remain unknown. In this study, we used RT-PCR to systematically investigate the expression of moesin in 49 astrocytomas of different pathological grade and 6 normal brain tissues. We found that the mRNA expression levels of moesin in astrocytomas were significantly higher in comparison with normal brain tissues. Furthermore, moesin up-regulation was correlated with pathological grade of astrocytomas. Subsequently, we tested 112 astrocytomas and 14 normal brain tissues by immunohistochemistry. Similar results were also confirmed. Univariate and multivariate survival analysis were used to determine the correlations of moesin expression with overall survival and progression-free survival. Our results showed the expression of moesin was strongly negatively correlated with the patient progression-free survival and overall survival. These results suggest moesin protein involved in the genesis and progression of astrocytomas and might be regarded as an independent predictor of poor prognosis.
Insights
Moesin protein expression is elevated in astrocytomas and linked to higher tumor grade. Increased moesin levels negatively correlate with patient survival, suggesting it as a poor prognostic marker in brain tumors.
Area of Science:
- Neuro-oncology
- Molecular Biology
- Cell Biology
Background:
- Moesin, an ERM family protein, links the actin cytoskeleton to the plasma membrane.
- It plays critical roles in cell morphology, motility, adhesion, and tumor progression.
- The role of moesin in astrocytoma pathogenesis and prognosis is currently unknown.
Purpose of the Study:
- To investigate the expression pattern of moesin in astrocytomas.
- To determine the clinical significance and prognostic value of moesin in astrocytoma patients.
Main Methods:
- RT-PCR was used to analyze moesin mRNA expression in 49 astrocytomas and 6 normal brain tissues.
- Immunohistochemistry was performed on 112 astrocytomas and 14 normal brain tissues.
- Univariate and multivariate survival analyses assessed the correlation between moesin expression and patient survival.
Main Results:
- Moesin mRNA and protein expression levels were significantly higher in astrocytomas compared to normal brain tissues.
- Moesin expression levels positively correlated with astrocytoma pathological grade.
- Higher moesin expression was strongly associated with reduced progression-free survival and overall survival.
Conclusions:
- Moesin is upregulated in astrocytomas and its expression correlates with tumor grade.
- Moesin expression is an independent predictor of poor prognosis in astrocytoma patients.
- Moesin protein may play a role in astrocytoma development and progression.
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