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Updated: May 15, 2026
Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
Published on: November 29, 2014
Purification of high-complexity peptide microarrays by spatially resolved array transfer to gold-coated membranes
Christopher Schirwitz1, Felix F Loeffler, Thomas Felgenhauer
1German Cancer Research Center (DKFZ), Functional Genome Analysis, Chip-based Peptide Libraries, Heidelberg, Germany. c.schirwitz@dkfz.de
Abstract:
A method for the one-step purification of high-complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full-length peptides covalently couple to the receptor membrane via an N-terminally added cysteine. Highly resolved peptide transfer and purification of up to 10 000 features per cm(2) is demonstrated.
