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Related Experiment Video

Updated: May 15, 2026

Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format
07:27

Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format

Published on: March 5, 2013

Generating regionalized neuronal cells from pluripotency, a step-by-step protocol.

Agnete Kirkeby1, Jenny Nelander, Malin Parmar

  • 1Department of Experimental Medical Science and Lund Stem Cell Center, Lund University Lund, Sweden.

Frontiers in Cellular Neuroscience
|January 15, 2013
PubMed
Summary
This summary is machine-generated.

Human pluripotent stem cells can be differentiated into neural cells for regenerative medicine and disease research. This protocol details neuralization and regionalization for creating specific neural subtypes.

Keywords:
GSK3brain developmentdifferentiationhuman embryonic stem cellsneuronal subtypespluripotent stem cellsprotocolregionalization

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Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons
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Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons

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The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells
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The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

Published on: April 14, 2013

Related Experiment Videos

Last Updated: May 15, 2026

Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format
07:27

Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format

Published on: March 5, 2013

Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons
11:52

Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons

Published on: August 26, 2021

The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells
10:49

The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

Published on: April 14, 2013

Area of Science:

  • Stem cell biology
  • Neuroscience
  • Regenerative medicine

Background:

  • Human pluripotent stem cells (hPSCs) offer potential for regenerative therapies in neurodegenerative diseases.
  • hPSCs are valuable for studying human neural development and modeling neurological disorders.
  • Recent advancements enable rapid and synchronized neural differentiation of hPSCs.

Purpose of the Study:

  • To present a detailed protocol for neuralization and regionalization of hPSCs.
  • To generate regionalized neural progenitors and subtype-specific neurons.
  • To facilitate transplantation studies and in vitro analysis of neural cells derived from hPSCs.

Main Methods:

  • Step-by-step protocol for neuralization of hPSCs.
  • Incorporation of positional patterning for regionalization.
  • Generation of subtype-specific neurons from differentiated hPSCs.

Main Results:

  • Successful neural conversion of hPSCs.
  • Efficient regionalization of neural progenitors.
  • Production of specific neuronal subtypes relevant to brain development.

Conclusions:

  • The presented protocol enables efficient generation of regionalized neural cells from hPSCs.
  • This method supports applications in regenerative medicine and disease modeling.
  • The protocol is suitable for both transplantation studies and in vitro analyses.