Related Experiment Video
Updated: May 15, 2026

Characterizing Lewis Pairs Using Titration Coupled with In Situ Infrared Spectroscopy
Published on: February 20, 2020
Development of an analytical approach to study a three-component hetero-association by means of spectrophotometry
Anatoly S Buchelnikov1, Alexander F Khrustalev, Maxim P Evstigneev
1Department of Physics, Sevastopol National Technical University, Sevastopol, 99053, Ukraine.
Abstract:
A case of 1:m:n complexation in a three-component system containing any possible heterocomplexes formed between the non-self-aggregating, absorbing ligand A and two self-aggregating, non-absorbing ligands B and C was considered for the first time in an application for molecular spectroscopy. All expressions necessary for full quantitative analysis of experimental data in three-component mixtures were obtained, viz., the law of conservation of mass and the expression for an experimentally observed parameter. These expressions can be directly utilized in mathematical software for performing standard curve-fitting procedures or solutions of specific tasks such as calculations of the concentration of various types of complexes. The numerical test of the 1:m:n model, accomplished with the aid of ultraviolet-visible light experimental data in a three-component system (proflavine-caffeine-nicotinamide), proved the validity of the developed approach.
Related Concept Videos
Spectrophotometry: Introduction
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Development of Analytical Methods
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Qualitative Analysis
There are two main approaches to qualitative analysis:...
Complexometric Titration: Overview
UV–Vis Spectroscopy of Conjugated Systems
One of the factors influencing λmax is the extent of conjugation in the...

