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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
Published on: December 12, 2019
Visualizing the location and the dynamics of gene expression in living animals through bioluminescence imaging
1Columbia Center for Translational Immunology, Columbia University College of Physicians and Surgeons, New York, NY 10032-3702, USA.
Methods in Cell Biology
|January 16, 2013
Abstract:
Bioluminescence imaging allows for real-time assessment of gene expression in vivo in models where luciferase expression is controlled by promoter elements of gene of interest. It provides a sensitive means of recording temporal and spatial resolution of gene expression. In this Chapter, protocols for the use of the bioluminescence imaging system in localizing and semi-quantitatively measuring gene expression in mice are discussed.

