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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
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Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Published on: March 13, 2018

Noninvasive molecular imaging using reporter genes.

Peter Brader1, Inna Serganova, Ronald G Blasberg

  • 1Department of Radiology, Division of Molecular Imaging, Medical University of Vienna, Vienna, Austria.

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
|January 16, 2013
PubMed
Summary
This summary is machine-generated.

Noninvasive reporter gene imaging uses molecular imaging to track biological processes in living subjects. This review highlights radionuclide-based systems for preclinical and clinical applications in disease monitoring and treatment evaluation.

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Area of Science:

  • Molecular Imaging
  • Biomedical Engineering
  • Radiochemistry

Background:

  • Noninvasive reporter gene imaging is a key component of molecular imaging.
  • It enables real-time assessment of biological processes in vivo.
  • Reporter imaging can utilize various imaging modalities.

Purpose of the Study:

  • This review focuses on radionuclide-based reporter gene imaging.
  • It examines the development and application of these systems in preclinical and clinical studies.
  • The review highlights human reporter systems and their applications.

Main Methods:

  • Review of existing literature on radionuclide-based reporter gene imaging.
  • Presentation of various reporter systems, emphasizing human systems.
  • Discussion of selected applications in disease research and treatment.

Main Results:

  • Radionuclide-based reporter gene imaging offers versatile applications.
  • Examples include adoptive cell therapies, gene/oncoviral therapies, oncogenesis, and signal pathway monitoring.
  • Imaging drug treatment efficacy is also a significant application.

Conclusions:

  • Noninvasive reporter gene imaging significantly advances understanding of disease.
  • It plays a crucial role in molecular medicine and personalized patient care.
  • Radionuclide-based systems are vital tools for in vivo biological process monitoring.