Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 15, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Upconversion nanoparticle based LRET system for sensitive detection of MRSA DNA sequence.

Jinliang Liu1, Jinting Cheng, Yong Zhang

  • 1Department of Bioengineering, Faculty of Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576.

Biosensors & Bioelectronics
|January 16, 2013
PubMed
Summary

We developed a sensitive and specific method to detect methicillin-resistant Staphylococcus aureus (MRSA) DNA. This technique utilizes upconversion nanoparticles (UCNs) and luminescence resonance energy transfer (LRET) for efficient DNA sequence identification.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Formation of an Amorphous LaAlBO<sub>x</sub> Overlayer on Crystalline LaAlO<sub>3</sub> Perovskite for Highly Efficient and Stable Propane Oxidative Dehydrogenation.

Angewandte Chemie (International ed. in English)·2026
Same author

Symmetric instability drives exchange between surface and bottom waters in a coastal front.

Science advances·2026
Same author

The Phase-Amplitude Coupling Induced by Drug Cues in Individuals With Methamphetamine Use Disorder During Withdrawal.

Brain and behavior·2026
Same author

Reinforcement Learning-Based Formation Control for Uncrewed Surface Vehicles Under Aperiodic DoS Attacks: A Stackelberg-Nash Game Approach.

IEEE transactions on cybernetics·2026
Same author

A 6G-Enabled Hierarchical Contrastive Learning Framework for Multi-Scale Medical Time Series Analysis.

IEEE journal of biomedical and health informatics·2026
Same author

A study on the mental health and body weight of Chinese college students in the context of COVID-19: implications for educational practice.

Frontiers in public health·2026

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Diagnostics

Background:

  • Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant public health threat.
  • Accurate and rapid detection of MRSA is crucial for effective treatment and infection control.
  • Existing diagnostic methods may have limitations in sensitivity, specificity, or speed.

Purpose of the Study:

  • To develop a novel, highly sensitive, and specific method for detecting MRSA DNA sequences.
  • To utilize upconversion nanoparticles (UCNs) as energy donors in a luminescence resonance energy transfer (LRET) assay.
  • To establish a versatile platform for pathogen DNA detection.

Main Methods:

  • Synthesis of citrate-capped NaYF4:Yb, Er upconversion nanoparticles (UCNs) dispersible in aqueous solutions.

More Related Videos

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
09:04

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Published on: September 17, 2017

Related Experiment Videos

Last Updated: May 15, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
09:04

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Published on: September 17, 2017

  • Covalent immobilization of MRSA capture oligonucleotides onto the UCN surface.
  • Development of a sandwich hybridization assay involving TAMRA-labeled report oligonucleotides and MRSA target DNA, leading to LRET.

    • Main Results:

    • Achieved specific detection of MRSA DNA sequences.
    • Demonstrated high sensitivity with a detection limit as low as 0.18 nM.
    • Successfully employed UCNs as energy donors in an efficient LRET process for DNA detection.

    Conclusions:

    • The developed UCN-LRET method provides an efficient and versatile approach for MRSA DNA detection.
    • This method offers high sensitivity and specificity, outperforming existing techniques.
    • This represents the first report of using UCNs as energy donors for MRSA DNA detection via LRET.