Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Development of the Oral Microbiota01:28

Development of the Oral Microbiota

The establishment of the oral microbiome begins before birth, challenging the long-held belief that the fetal oral cavity is sterile. The presence of oral microbes such as Streptococcus and Fusobacterium in amniotic fluid suggests that microbial exposure may occur in utero, potentially through translocation from the maternal oral or gastrointestinal tract. This early colonization primes the neonatal immune system and sets the stage for subsequent microbial succession. Maternal health,...
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
The Oral Microbiota01:27

The Oral Microbiota

The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the lytic replication...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Biofilms01:29

Biofilms

Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gingival and Periodontal Diseases and Conditions in Children and Adolescents: Consensus Report.

Journal of clinical periodontology·2026
Same author

Gingival and periodontal diseases and conditions in children and adolescents: consensus report.

European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry·2026
Same author

Association between vitamin D supplementation and the prevalence of dental caries and gingivitis in children. A cross-sectional study.

Clinical oral investigations·2025
Same author

Probiotics, Prebiotics, Synbiotics, and Postbiotics Against Oral Candida in Children: A Review of Clinical Trials.

Nutrients·2025
Same author

Fluoride mouthrinses for prevention of initial caries in orthodontic patients - a systematic review and meta-analysis.

BMC oral health·2025
Same author

Erosive Tooth Wear in Children and Adolescents: Awareness, Knowledge, and Management: A Case-Based Questionnaire Among Greek Dentists.

Dentistry journal·2025

Related Experiment Video

Updated: May 15, 2026

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries
08:20

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Published on: March 31, 2021

Are we ready for caries prevention through bacteriotherapy?

Svante Twetman1

  • 1Department of Odontology, Section for Cariology, Endodontics, Pediatric Dentistry and Clinical Genetics, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. stwe@sund.ku.dk

Brazilian Oral Research
|January 16, 2013
PubMed
Summary

Probiotic bacteria show promise in modulating oral biofilms and potentially preventing cavities. Further large-scale clinical trials are needed to confirm bacteriotherapy

More Related Videos

Adjunctive Diode Laser Therapy and Probiotic Lactobacillus Therapy in the Treatment of Periodontitis and Peri-Implant Disease
06:06

Adjunctive Diode Laser Therapy and Probiotic Lactobacillus Therapy in the Treatment of Periodontitis and Peri-Implant Disease

Published on: May 9, 2022

Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria
09:57

Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria

Published on: December 14, 2016

Related Experiment Videos

Last Updated: May 15, 2026

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries
08:20

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Published on: March 31, 2021

Adjunctive Diode Laser Therapy and Probiotic Lactobacillus Therapy in the Treatment of Periodontitis and Peri-Implant Disease
06:06

Adjunctive Diode Laser Therapy and Probiotic Lactobacillus Therapy in the Treatment of Periodontitis and Peri-Implant Disease

Published on: May 9, 2022

Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria
09:57

Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria

Published on: December 14, 2016

Area of Science:

  • Microbiology and Oral Health
  • Bacteriotherapy and Probiotics

Background:

  • Human biofilms are crucial for health, leading to interest in microbiota modulation via bacteriotherapy.
  • In dentistry, probiotic bacteria are being explored for their potential to stabilize and diversify oral biofilms.
  • Caries prevention is a key focus, with bacterial interference strategies gaining attention.

Purpose of the Study:

  • To investigate the potential role of probiotic lactobacilli and bifidobacteria in modulating oral microbial ecology.
  • To assess the efficacy of probiotic interventions in interfering with caries-associated bacteria.
  • To evaluate the preventive effects of probiotic supplementation on caries outcomes.

Main Methods:

  • In vitro investigations assessed metabolic activity, co-aggregation, growth inhibition, bacteriocin production, and adhesion of probiotic bacteria.
  • Short-term clinical studies examined intermediate microbial endpoints.
  • Randomized controlled clinical trials with caries outcomes were reviewed.

Main Results:

  • In vitro studies suggest probiotic lactobacilli and bifidobacteria can modulate oral microbial ecology.
  • Short-term clinical studies indicate potential interference with caries-associated bacteria through various probiotic delivery forms.
  • Three studies in preschool children and the elderly showed caries preventive fractions of 21-75% with probiotic-supplemented milk.

Conclusions:

  • Probiotic bacteria, particularly lactobacilli and bifidobacteria, show potential for oral health applications.
  • Evidence suggests bacteriotherapy may complement existing caries prevention methods.
  • Larger-scale clinical trials are necessary to validate orally derived anti-caries candidates for daily practice.